Dr. S.V. Balasubramanian, 541 Cooke Hall, 645-2844 x245 (svb@acsu.buffalo.edu)
This course consists of lectures and demonstrations/MiniLabs. Introduction to biotechnology derived products and genomics, protein/peptide and gene structure and sequence search, the formulation and delivery of proteins/peptides and gene based biopharmaceuticals, and the methods for their analysis, will provide an extended theme for the course.
Topics include protein and peptide formulation, with emphasis on the importance of maintaining protein structure/conformation, physical and chemical stability, and the role of lyophilization and cryoprotectants. The course will emphasize analytical methods to study chemical stability (amino acid sequencing, mass spectroscopy) and physical stability (aggregation and precipitation), along with physical methods to study physical stability (Circular Dichroism (CD), fluorescence), peptide conformation, and transport. Laboratory demonstrations will include exercises in protein formulation by lyophilization, and evaluation of physical stability using CD, fluorescence and turbidity measurements.
The class is scheduled to meet on Tuesdays between 2.30 - 3:40 pm in Cooke 508. For lab sessions class will meet in Pharmaceutical Sciences Instrumentation facility between 2.30pm and 5.30pm*.
|
Date |
Topic |
Pages |
Instructor (s) |
|
1/18/2000 |
Course overview |
2&3 |
Dr Balu Pharmaceutics |
|
1/25/2000 |
Pharmaceutics and protein drugs |
4-28 |
Drs. Balu and Straubinger, Pharmaceutics |
|
2/1/2000 |
Protein structure and forces in proteins |
29-33 |
Dr Srikrishnan RPCI |
|
2/3/2000 # |
Introduction to Biotechnology |
TBA |
|
|
2/8/2000 |
Recombinant DNA technology |
34-41 |
Dr Gollnick, Biology |
|
2/15/2000 |
Computational Biophysics, Bioinformatics, Genomics and proteomics |
42-48 |
Dr Balu, Pharmaceutics Dr M.S.Rao, Glycodesign Inc. |
|
2/22/2000 |
Protein structure determination |
49-52 |
Dr Balu Pharmaceutics |
|
2/29/2000 |
Chemical Stability of protein drugs |
53-70 |
Dr Balu Pharmaceutics |
|
3/14/2000/2/1/2000 |
Determination of chemical stability |
71-77 |
TBA / Dr Wood, Chemistry |
|
3/21/2000 |
Freeze drying of proteins |
78-88 |
Dr Balu and TBA (Scientist from Baxter, Hyland Immuno) |
|
3/23/2000 |
Formulation of Protein drugs |
Dr T.Nguyen, Genentech, CA |
|
|
3/28/2000 |
Physical stability and protein folding |
89-104 |
Dr Balu Pharmaceutics |
|
4/4/2000 |
Formulation development strategies (+delivery) |
105-123 |
Dr Balu Pharmaceutics |
|
4/11/2000 |
Biotechnology, pharmaceutics of gene based drugs |
124-126 |
Dr Balu Pharmaceutics |
|
4/18/2000 |
Structure of nucleic acids |
Dr Eliseev Chemistry |
|
|
4/25/2000 |
Formulation, delivery and stability of gene based drugs |
127-131 |
Drs. Balu and Straubinger Pharmaceutics |
#
Special Seminar ClassesMinilabs:
|
Date |
Topic |
Pages |
Instructor(s) |
|
2/8/2000 |
Introduction to Protein data bank |
132 |
Dr Balu Pharmaceutics |
|
2/15/2000 |
Bioinformatics |
Dr Balu Pharmaceutics Dr M.S.Rao, Glycodesign Inc. |
|
|
2/22/2000 |
Secondary structure of proteins, CD |
137-149 |
Dr Balu Pharmaceutics |
|
2/29/2000 |
" |
" |
Dr Balu Pharmaceutics |
|
3/14/2000 |
Tertiary structure, Fluorescence |
150-156 |
Dr Balu Pharmaceutics |
|
3/21/2000 |
Molecular Modeling |
Dr Balu Pharmaceutics Dr Srikrishnan, RPCI |
|
|
Lab demonstration (TBA) |
FT IR |
157-161 |
Dr Sen RPCI |
|
Lab demonstration (TBA) |
Quasi elastic light scattering and protein aggregation |
162-165 |
Dr Sen RPCI |
*
On some days both lecture and lab sessions are scheduled around the same time. On those days lab will follow the lectures.
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